Turn down - turn up - a simple and low-cost protocol for preparing platelet-rich plasma

Abstract - OBJECTIVE:To describe and analyze a new protocol for the extraction of platelet-rich plasma (PRP) for use inclinical practice and compare this technique with methods that have been previously described in the medicalliterature.METHODS:We extracted PRP from 20 volunteers using four different protocols (single spin at 1600g, singlespin at 600g, double spin at 300 and 700g, and double spin at 600 and 900g). In another group of12 individuals, we extracted PRP with our new technique (named‘turn down-turn up’) consisting of a doublespin (200gand1600g) closed system using standard laboratory equipment (including an ordinary benchtopcentrifuge), where the blood remained in the same tube during all processes, reducing the risk of contamination.Platelet counts adjusted to baseline values were compared using analysis of covariance (ANCOVA).RESULTS:Using the four previously described protocols (mentioned above), we obtained concentrations ofplatelets that were 1.15-, 2.07-, 2.18-, and 3.19-fold greater than the baseline concentration, respectively. Withthe turn down-turn up technique, we obtained a platelet count that was 4.17-fold (95% confidence interval (CI):3.09 to 5.25) greater than the baseline platelet count (p=0.063 compared with the double spin at 600 and900g method). The total cost of the disposable materials used in the extraction process was less thanUS$10.00 per individual. CONCLUSION:In the present study, we described a simple and safe method for obtaining PRP using low-costdevices. KEYWORDS:Platelet-Rich Plasma; Regenerative Medicine; Orthopedics.